2022 ILSI Federation Scholar Travel Awardees

2022 ILSI Federation Scholar Travel Awardees

The ILSI Federation Scholar Travel (IFST) Award is granted to exceptional early career scientists to help foster professional growth and development.

Session details: IFST – Early Career Scientist Talks and Poster Session 

Monday, June 27, 2:40-3:45 p.m. CEST

Tuesday, June 28, 5:30 – 7:00 p.m. CEST

Topics covered by each presenter:

Presenters and awardees:

Subrota Hati

Influence of dietary supplementation of Lactobacillus and Saccharomyces cultures on growth performance, fecal microbiota, blood profile, cholesterol contents and metagenomic study by replacing antibiotics as growth promoter in broilers - Dr. Subrota Hati, Assistant Professor at the Department of Dairy Microbiology, SMC College of Dairy Science, Kamdhenu University, India

Abstract: The study is aimed to optimize the growth conditions for the production of physiologically active peptides from fermented milks using probiotic lactic cultures and to separate, purify and characterize the novel peptides from fermented goat, camel, bovine and soy milks of Indian breeds for the wellbeing of the hosts. These fermented milks help to prevent then lifestyle diseases. The study is for the improvement of health and nutritional potential of traditional Indian fermented dairy foods through biotechnological interventions. Bioactive peptides with antihypertensive (ACE inhibitory), antioxidative, antimicrobial potential have been identified and characterized from fermented milks of camel, bovine, goat and soy. We have started to purify and characterize the bioactive peptides from different fermented milks (bovine, camel and goat of Indian origin) with potent indigenous well identified probiotic cultures viz., L. rhamnosus MTCC 5945 (NS4), L. rhamnosus MTCC 5946 (NS6), L. acidophilus NCDC (298), L. helveticus MTCC 5463 (V3), S. thermophilus MTCC 5460 (MD2), Lactococcus lactis subsp. lactis MTCC 25066 (NK6), L. fermentum TDS030603 (LBF). Their suitability in product manufacture was also evaluated by studying their growth pattern in milks, by measuring rate of acid production, pH and bacterial counts and other physico-chemical parameters. Fermented camel milk was prepared using proteolytic Lactobacillus rhamnosus MTCC 5945 (NS4), which was analyzed for X-prolyl-dipeptidyl aminopeptidase activity and Angiotensin I-Converting Enzyme (ACE) inhibitory activity. Fractionated 3 kDa, 5 kDa and 10 kDa permeate and retentate samples were further analyzed for ACE-inhibitory activity under the optimized growth conditions. Subsequently, we have isolated and purified the novel peptides with free radical scavenging activity, antioxidative activity and ACE-inhibitory activity, antimicrobial and antidiabetic activities through in vitro and in vivo studies and also developed functional fermented milks with these activities.

The influence of dietary supplementation of Lactobacillus cultures on growth performance, fecal microbiota, blood profile and cholesterol contents by replacing antibiotics as growth promoter in broilers with increasing concerns about antibiotic resistance was studied as well. There is increasing interest in finding alternatives to antibiotics for poultry production. Feeding of strong lactic acid bacteria with proven probiotic attributes could be an alternative for the replacement of antibiotics in poultry feeds for the production of antibiotic free eggs and meat production. In the present study, Two Lactobacillus cultures i.e. L. plantarum (KGL3A, GN: MF951099) and L. fermentum (KGL4, GN: MG722814) were considered as an alternative to growth promoter (Antibiotics). Whole genome sequence of L. plantarum (KGL3A, GI: MF951099) and L. fermentum (KGL4, GI: MG722814) were submitted in the NCBI. Lactic acid bacteria could be an alternative for antibiotic free meat and egg production in future.

Dr. Subrota Hati is an Assistant Professor in the Department of Dairy Microbiology, SMC College of Dairy Science, Kamdhenu University, Anand, Gujarat, India. Dr. Hati obtained his Doctorate Degree in dairy microbiology from the National Dairy Research Institute, Karnal, India (2008-2012). Dr. Hati completed his post-doctoral study at The University of Queensland, School of Agriculture and Food Sciences, Australia. Dr. Hati’s research focuses on producing antibiotic free meat and eggs from broilers using dietary supplements with well characterized probiotic cultures. He is also exploring the biofunctionalities of novel peptides through fermentation using potent indigenous Lactic acid bacteria with probiotic potential to promote the health benefits of the consumers.

Kelsey M. Mangano
Boushra Dalile
Tulio Konstantyner
Dahiana Quesada
Wei-Hsiang Chang
S. Kokilavani