2022 ILSI Federation Scholar Travel Awardees
The ILSI Federation Scholar Travel (IFST) Award is granted to exceptional early career scientists to help foster professional growth and development.
Session details: IFST – Early Career Scientist Talks and Poster Session
Monday, June 27, 2:40-3:45 p.m. CEST
Tuesday, June 28, 5:30 – 7:00 p.m. CEST
Topics covered by each presenter:
Presenters and awardees:
Diet-derived fruit and vegetable metabolites show sex-specific inverse relationsihps to osteoporosis status - Kelsey M. Mangano, Associate Professor at the Department of Biomedical and Nutritional Sciences, University of Massachusetts, Lowell, USA
Abstract: Background: The influence of nutrition on the metabolic profile of osteoporosis (OS) is unknown. Objective: Identify biochemical factors driving the association of fruit and vegetable (FV) intakes with OS prevalence using an untargeted metabolomics approach. Design: Cross-sectional dietary, anthropometric and plasma metabolite data were examined from the Boston Puerto Rican Osteoporosis Study, n=600 (46-79yr). Methods: Bone mineral density was assessed by DXA. OS was defined by clinical standards. A culturally adapted FFQ assessed usual dietary intake. Principal components analysis (PCA) of 42 FV items created 6 factors. Metabolomic profiles derived from plasma samples were assessed on a commercial platform. Differences in levels of 525 plasma metabolites between disease groups (OS vs no-OS) were compared using logistic regression; and associations with FV intakes by multivariable linear regression, adjusted for covariates. Metabolites significantly associated with OS status or with total FV intake were analyzed for enrichment in various biological pathways using Mbrole 2.0, MetaboAnalyst, and Reactome, using FDR correction of P-values. Correlation coefficients were calculated as Spearman’s rho rank correlations, followed by hierarchical clustering of the resulting correlation coefficients using PCA FV factors and sex-specific sets of OS-associated metabolites. Results: High FV intake was inversely related to OS prevalence (Odds Ratio = 0.73; 95% CI = 0.57, 0.94; P = 0.01). Several biological processes affiliated with the FV-associating metabolites, including caffeine metabolism, carnitines and fatty acids, and glycerophospholipids. Important processes identified with OS-associated metabolites were steroid hormone biosynthesis in women and branched-chain amino acid metabolism in men. Factors derived from PCA were correlated with the OS-associated metabolites, with high intake of dark leafy greens and berries/melons appearing protective in both sexes. Conclusions: These data warrant investigation into whether increasing intakes of dark leafy greens, berries and melons causally affect bone turnover and BMD among middle-aged and older adults at risk for osteoporosis via sex-specific metabolic pathways, and how gene-diet interactions alter these sex-specific metabolomic-osteoporosis links.
Kelsey M. Mangano, Ph.D., R.D., is Associate Professor and Director of Nutritional Science at The University of Massachusetts, Lowell. She is a member of the Center for Population Health at UML and Center for Microbiome Research, UMass Medical School. Professor Mangano’s research lab aims to elucidate mechanisms behind the impact of nutritional factors and food additives on musculoskeletal aging and the gut. Current mechanisms include alterations in the gut microbiome and metabolomic response to nutrition.
For more information and contact details: Google Scholar Profile, Faculty Website, Twitter: @ManganoKelsey